RNA

Part:BBa_K2087000:Design

Designed by: Nikhil Nair   Group: iGEM16_Tufts   (2016-10-14)


CRISPR/Cas9 sgRNA Targeting Locus 618 on the DD96 Human Gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1012
    Illegal XhoI site found at 1904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 polymerase restricted.

Source

BBa_K1818000 (Tufts iGEM Team 2015)

References